abstract：:Strains of Bacillus subtilis are plant growth-promoting bacteria (PGPB) of many crops and are used as inoculants. PGPB colonization is an important trait for success of a PGPB on plants. A specific probe, based on the 16 s rRNA of Bacillus subtilis, was designed and evaluated to distinguishing, by fluorescence in situ...

journal_title：Journal of microbiological methods

authors： Posada LF,Alvarez JC,Hu CH,de-Bashan LE,Bashan Y

更新日期：2016-09-01 00:00:00

abstract：:Cell wall glycopolymers (CWG) represent an important component of the Gram-positive cell envelope with many biological functions. The mycelial soil bacterium Streptomyces coelicolor A3(2) incorporates two distinct CWGs, polydiglycosylphosphate (PDP) and teichulosonic acid, into the cell wall of its vegetative mycelium...

journal_title：Journal of microbiological methods

authors： Sigle S,Steblau N,Wohlleben W,Muth G

更新日期：2016-09-01 00:00:00

abstract：:The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and ...

journal_title：Journal of microbiological methods

authors： Bettarel Y,Thanh MC,Patrice G,Antoinette A,Nadège KN,Bui VN,Thierry B

更新日期：2016-09-01 00:00:00

abstract：:Leishmaniasis is a disease, caused by Leishmania parasites, which infect humans and animals, posing a major social and economic burden worldwide. The need for accurate and sensitive disease diagnosis led to the widespread adoption of PCR amplification. Detection of the amplification products (i.e. gel electrophoresis)...

journal_title：Journal of microbiological methods

authors： Toubanaki DK,Athanasiou E,Karagouni E

更新日期：2016-08-01 00:00:00

abstract：:MALDI-ToF MS can be used to identify microorganisms directly from blood cultures. This study compared two methods of sample preparation. Similar levels of genus- (91% vs 90%) and species-level identifications (79% vs 74%) were obtained with differential centrifugation and SDS methods. The SDS method is faster and requ...

journal_title：Journal of microbiological methods

authors： Robinson AM,Ussher JE

更新日期：2016-08-01 00:00:00

abstract：:The present study describes the development of a simple and efficient screening system that allows identification and quantification of nine bacteriocins produced by Lactococcus lactis. Cell-free L. lactis extracts presented a broad spectrum of antibacterial activity, including Gram-negative bacteria, Gram-positive ba...

journal_title：Journal of microbiological methods

authors： Katharopoulos E,Touloupi K,Touraki M

更新日期：2016-08-01 00:00:00

abstract：:Streptococcus gordonii, a commensal bacterium of the human oral cavity, is a potential live vaccine vector. In this study, we have developed a system that delivers a vaccine antigen gene onto the chromosome of S. gordonii. The system consisted of a recipient strain, that is a thymidine auxotroph constructed by deletio...

journal_title：Journal of microbiological methods

authors： Lee SF,Hulbah M,Halperin SA

更新日期：2016-06-01 00:00:00

abstract：:In this research work, the synthesis of Ag doped hydroxyapatite coatings for dental or orthopedic implants was performed. The main goal was to determine the influence of Ag content on the roughness and antimicrobial performance of the prepared thin films. The films were deposited on Ti6Al4V alloy by means of RF magnet...

journal_title：Journal of microbiological methods

authors： Ciuca S,Badea M,Pozna E,Pana I,Kiss A,Floroian L,Semenescu A,Cotrut CM,Moga M,Vladescu A

更新日期：2016-06-01 00:00:00

abstract：:We developed an effective and rapid assay to detect both bio-energetic and envelope permeability (BEEP) alterations of Pseudomonas aeruginosa. The assay is based on quantification of extracellular ATP in bacterial cultures using luciferase as a reporter. To demonstrate the validity of our assay we conducted a biased s...

journal_title：Journal of microbiological methods

authors： Suh SJ,Shuman J,Carroll LP,Silo-Suh L

更新日期：2016-06-01 00:00:00

abstract：:In this study, we aimed to identify the molecular carbapenemase types of the Enterobacteriaceae isolates and to evaluate the performance of manually prepared and commercially available combination disc methods and the modified Hodge test. One hundred and forty carbapenemase producing isolates and 45 isolates as contro...

journal_title：Journal of microbiological methods

authors： Genc O,Aksu E,Gulcan A

更新日期：2016-06-01 00:00:00

abstract：:The lack of a surrogate-of-immunity assay presents a major barrier to Streptococcus pyogenes research. Modification of the Lancefield assay to include an antibody digestion step reduced inter-donor variation and permitted detection of the anti-streptococcal activity of intravenous immunoglobulin and convalescent serum...

journal_title：Journal of microbiological methods

authors： Reglinski M,Lynskey NN,Sriskandan S

更新日期：2016-05-01 00:00:00

abstract：:To ensure quality management during the production processes of probiotics and for efficacy testing in vivo, accurate tools are needed for the identification and quantification of probiotic strains. In this study, a strain-specific qPCR assay based on Suppression Subtractive Hybridisation (SSH) for identifying unique ...

journal_title：Journal of microbiological methods

authors： Fibi S,Klose V,Mohnl M,Weber B,Haslberger AG,Sattler VA

更新日期：2016-04-01 00:00:00

abstract：:Immunoproteomic technology offers an exceptional tool to fill the blanks that still exist in diagnosis of Toxoplasma gondii infection despite its annotated sequence. The pitfalls of serological assays and current immunoproteomic approaches are accentuated, and new approaches are presented to improve the signal and to ...

journal_title：Journal of microbiological methods

authors： El-Ashram S,Yin Q,Barta JR,Khan J,Liu X,Suo X

更新日期：2015-12-01 00:00:00

abstract：:Culture in enriched broth, as well as on a solid medium, is recommended for primary isolation of mycobacteria. With the introduction of liquid mycobacterial culture methods, a substantial workload regarding the identification of culture-recovered mycobacterial species, particularly Mycobacterium tuberculosis complex (...

journal_title：Journal of microbiological methods

authors： Kim JU,Cha CH,Park SH

更新日期：2015-12-01 00:00:00

abstract：:Laccase is a key enzyme in the degradation of lignin by fungi. Reports indicate that the activity of this enzyme ranges from 3.5 to 484,000 U L(-1). Our aim was to analyze how laccase activity is calculated in the literature, and to determine statistically whether variations in activity are due to biological propertie...

journal_title：Journal of microbiological methods

authors： Baltierra-Trejo E,Márquez-Benavides L,Sánchez-Yáñez JM

更新日期：2015-12-01 00:00:00

abstract：:O157, O26, and O111 are the most important O serogroups of enterohemorrhagic Escherichia coli worldwide. Recently we reported a strategy for discriminating these serotypes from the others using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) based on the S10-spc-alpha operon...

journal_title：Journal of microbiological methods

authors： Ojima-Kato T,Yamamoto N,Iijima Y,Tamura H

更新日期：2015-12-01 00:00:00

abstract：:Antimicrobial susceptibility testing of Campylobacter isolates is of great importance for treatment options especially in systemic diseases. The European Committee for Antimicrobial Susceptibility Testing (EUCAST) recently proposed epidemiological cut-offs (ECOFFs) for a limited number of antimicrobial compounds and f...

journal_title：Journal of microbiological methods

authors： Sifré E,Salha BA,Ducournau A,Floch P,Chardon H,Mégraud F,Lehours P

更新日期：2015-12-01 00:00:00

abstract：:Contaminated eukaryotic cell cultures are frequently responsible for unreliable results. Regulatory entities request that cell cultures must be mycoplasma-free. Mycoplasma contamination remains a significant problem for cell cultures and may have an impact on biological analysis since they affect many cell parameters....

journal_title：Journal of microbiological methods

authors： Falagan-Lotsch P,Lopes TS,Ferreira N,Balthazar N,Monteiro AM,Borojevic R,Granjeiro JM

更新日期：2015-11-01 00:00:00

abstract：:A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given t...

journal_title：Journal of microbiological methods

authors： Tienaho J,Sarjala T,Franzén R,Karp M

更新日期：2015-11-01 00:00:00

abstract：:A 384-well-based antibacterial assay amenable for high-throughput screening and combination testing is described. The assay uses 100-500nL of test compounds and tolerates up to 2.5% dimethyl sulfoxide concentrations. It can be used for screening compound libraries and testing combinatory/synergistic/antagonistic effec...

journal_title：Journal of microbiological methods

authors： Mishra A,Dobritsa SV,Crouch ML,Rabenstein J,Lee JX,Dhakshinamoorthy S

更新日期：2015-11-01 00:00:00

abstract：:A variety of protein expression tags with different biochemical properties has been used to enhance the yield and solubility of recombinant proteins. Ubiquitin, SUMO (small ubiquitin-like modifier) and prokaryotic ubiquitin like MoaD (molybdopterin synthase, small subunit) fusion tags are getting more popular because ...

journal_title：Journal of microbiological methods

authors： Varga S,Pathare GR,Baka E,Boicu M,Kriszt B,Székács A,Zinzula L,Kukolya J,Nagy I

更新日期：2015-11-01 00:00:00

abstract：:Several studies have been conducted to develop rapid methods for quantification of lipid content in microalgae, as an alternative for time consuming gravimetric methods. Different studies showed that lipid staining with Nile Red in whole cell suspensions and subsequently quantification by the use of a spectrofluoromet...

journal_title：Journal of microbiological methods

authors： Balduyck L,Veryser C,Goiris K,Bruneel C,Muylaert K,Foubert I

更新日期：2015-11-01 00:00:00

abstract：:The U.S. Environmental Protection Agency's (EPA) Water Laboratory Alliance (WLA) currently uses ultrafiltration (UF) for concentration of biosafety level 3 (BSL-3) agents from large volumes (up to 100-L) of drinking water prior to analysis. Most UF procedures require comprehensive training and practice to achieve and ...

journal_title：Journal of microbiological methods

authors： Mapp L,Klonicki P,Takundwa P,Hill VR,Schneeberger C,Knee J,Raynor M,Hwang N,Chambers Y,Miller K,Pope M

更新日期：2015-11-01 00:00:00

abstract：:We report the development and evaluation of a serotype-specific xTAG luminex assay (SSA) that allows detection of the 33 serotypes of Streptococcus suis (S. suis). This assay is based on wzy gene targets directly involved in the cps biosynthesis and can be completed 40 min post-PCR amplification. The assay correctly a...

journal_title：Journal of microbiological methods

authors： Bai X,Liu Z,Ji S,Gottschalk M,Zheng H,Xu J

更新日期：2015-10-01 00:00:00

abstract：:Xylella fastidiosa is a member of the gamma proteobacteria. It is fastidious, insect-vectored and xylem-limited and causes a variety of diseases, some severe, on a wide range of economically important perennial crops, including grape and citrus. Antibody based detection assays are commercially available for X. fastidi...

journal_title：Journal of microbiological methods

authors： Yuan Q,Jordan R,Brlansky RH,Istomina O,Hartung J

更新日期：2015-10-01 00:00:00

abstract：UNLABELLED:An important goal for the improved diagnosis and management of infectious and inflammatory diseases, such as periodontitis, is the development of rapid and accurate technologies for the decentralized detection of bacterial pathogens. The aim of this prospective multicenter study was to evaluate the clinical ...

journal_title：Journal of microbiological methods

authors： Imamura K,Takayama S,Saito A,Inoue E,Nakayama Y,Ogata Y,Shirakawa S,Nagano T,Gomi K,Morozumi T,Akiishi K,Watanabe K,Yoshie H

更新日期：2015-10-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are important for biodegradable plastic production, and prokaryotes play a very important role in PHA production. PHA synthase is a key enzyme for the polymerization of PHAs. There are four classes of PHA synthase. The phaC gene is necessary for the production of all classes of PHA synthas...

journal_title：Journal of microbiological methods

authors： Muangsuwan W,Ruangsuj P,Chaichanachaicharn P,Yasawong M

更新日期：2015-09-01 00:00:00

abstract：:To rapidly and simply determine whether or not bacterial colonies growing on agar were Vibrio parahaemolyticus, we developed an immunochromatographic assay (VP-ICA) using two different monoclonal antibodies (designated mAb-VP34 and mAb-VP109) against the delta subunit of V. parahaemolyticus-F0F1 ATP synthase. The epit...

journal_title：Journal of microbiological methods

authors： Sakata J,Kawatsu K,Iwasaki T,Kumeda Y

更新日期：2015-09-01 00:00:00

abstract：:Filamentous fungi have shown great potential in the pretreatment of lignocellulosic biomass and their use in bio-processes based on Solid State Fermentation (SSF) opens promising perspectives for plant biomass valorization. Obviously, quantification of the fungal biomass throughout the fermentation is a crucial parame...

journal_title：Journal of microbiological methods

authors： Simeng Z,Sacha G,Isabelle HG,Marie-Noëlle R

更新日期：2015-08-01 00:00:00

abstract：:Bacterial metabolic activity is associated with the onset and progression mechanisms of oral biofilm-mediated disease; however, at present no method to monitor bacterial metabolism exists, especially for periodontopathic bacteria. Therefore, we aimed to establish a novel method for monitoring the metabolic activity of...

journal_title：Journal of microbiological methods

authors： Ishiguro K,Washio J,Sasaki K,Takahashi N

更新日期：2015-08-01 00:00:00

abstract：:In this study, a simple and rapid method was developed to transform protein A producing Staphylococcus aureus cells into red color and red fluorescent nanobioparticles, which were homogeneous, dispersive and relatively stable with a uniform size of 800 nm. The method consists of reaction with a monotetrazolium redox d...

journal_title：Journal of microbiological methods

authors： Hu W,Zhang Y,Yang H,Yu J,Wei H

更新日期：2015-08-01 00:00:00

abstract：:A fused protein composed of a carbohydrate-binding module and green fluorescence protein (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage of the GFP part of this protein using a site-specific protease allowed for the non-invasive and quantitative eva...

journal_title：Journal of microbiological methods

authors： Ojima Y,Suparman A,Nguyen MH,Sakka M,Sakka K,Taya M

更新日期：2015-07-01 00:00:00

abstract：:We evaluated the performance of two MALDI-TOF MS systems for the identification of clinically important Gram-positive cocci. Vitek MS and Microflex LT correctly identified 97.2% and 94.7%, respectively. Both systems offer reliable and rapid identification of clinically important Gram-positive cocci isolated in clinica...

journal_title：Journal of microbiological methods

authors： Lee M,Chung HS,Moon HW,Lee SH,Lee K

更新日期：2015-06-01 00:00:00

abstract：:A novel in vitro onychomycosis model was developed to easily predict the topical activity potential of novel antifungal drugs. The model encompasses drug activity and diffusion through bovine hoof slices in a single experimental set-up. Results correspond well with the antifungal susceptibility assay and Franz cell di...

journal_title：Journal of microbiological methods

authors： Sleven R,Lanckacker E,Boulet G,Delputte P,Maes L,Cos P

更新日期：2015-05-01 00:00:00

abstract：:The Baranyi and Roberts model is one of the most frequently used microbial growth models. It has been successfully applied to numerous studies of various microorganisms in different food products. Under dynamic conditions, the model is implicitly formulated as a set of two coupled differential equations which could be...

journal_title：Journal of microbiological methods

authors： Zhu S,Chen G

更新日期：2015-05-01 00:00:00

abstract：:Host DNA may adversely affect metagenomic studies focusing on the prokaryotic microbiota. This study compared the levels of host DNA in subgingival plaque collected by paper points and curette, using quantitative PCR. Lower proportions of host DNA and higher proportions of bacterial DNA were recovered from samples col...

journal_title：Journal of microbiological methods

authors： Pérez-Chaparro PJ,Duarte PM,Pannuti CM,Figueiredo LC,Mestnik MJ,Gonçalves CP,Faveri M,Feres M

更新日期：2015-04-01 00:00:00

abstract：:The reproducibility and reliability of quantitative microbiological assessments using the DNA-DNA hybridization "checkerboard method" (CKB) were assessed. The data originated from 180 chronic periodontitis patients, who were enrolled in a clinical trial and sampled at baseline, and 3 and 12m post-therapy. The samples ...

journal_title：Journal of microbiological methods

authors： Dahlen G,Preus HR,Baelum V

更新日期：2015-03-01 00:00:00

abstract：:Culture-independent studies using next generation sequencing have revolutionized microbial ecology, however, oomycete ecology in soils is severely lagging behind. The aim of this study was to improve and validate standard techniques for using high throughput sequencing as a tool for studying oomycete communities. The ...

journal_title：Journal of microbiological methods

authors： Sapkota R,Nicolaisen M

更新日期：2015-03-01 00:00:00

abstract：:Small-colony variants (SCVs) are slow-growing subpopulations of various auxotrophic bacterial strains. Thymidine-dependent SCVs (TD-SCVs) are unable to synthesize thymidine; hence, these variants fail to grow in a medium without thymidine. In this study, we used 10 TD-SCVs of Staphylococcus aureus, of which four strai...

journal_title：Journal of microbiological methods

authors： Horiuchi K,Matsumoto T,Ota Y,Kasuga E,Negishi T,Yaguchi T,Sugano M,Honda T

更新日期：2015-03-01 00:00:00

abstract：:Actinomyces naeslundii is an early colonizer with important roles in the development of the oral biofilm. The effects of butyric acid, one of short chain fatty acids in A. naeslundii biofilm formation was observed using a flow cell system with Tryptic soy broth without dextrose and with 0.25% sucrose (TSB sucrose). Si...

journal_title：Journal of microbiological methods

authors： Arai T,Ochiai K,Senpuku H

更新日期：2015-02-01 00:00:00